| A&P Falmouth Ltd | Cornwall |  |
| Aardman Animations Limited | Bristol | |
| Acheson Construction | Dorset | |
| Adido Solutions Limited | Dorset | |
| AGD Systems Limited | Gloucestershire | |
| Air Control Industries Ltd | Somerset | |
| Aish Technologies Ltd | Dorset | |
| Aldermans | Devon | |
| Allan Webb Ltd | Gloucestershire | |
| Allen and York Ltd | Dorset | |
| Amberley Adhesive Labels Limited | Dorset | |
| Amsafe Bridport | Dorset | |
| AP Valves | Cornwall | |
| Aquarium Technology Limited | Dorset | |
| Arcol UK Limited | Cornwall | |
| Ashfords | Devon | |
| Aster Group | Wiltshire | |
| Aurora Care Limited | Somerset | |
| Avon Metals Ltd | Gloucestershire | |
| Axiom Veterinary Laboratories Limited | Devon | |
| Aylesworth Fleming | Dorset | |
| Babcock Integrated Technology | Bristol | |
| Bandvulc Group | Devon | |
| Beacon South West | Devon | |
| Beard Limited | Wiltshire | |
| Bedruthan Steps Hotel Ltd | Cornwall | |
| BIBIC (British Inst. for Brain Injured Children) | Somerset | |
| Bishop Fleming | Devon | |
| Bott Limited | Cornwall | |
| Bottlegreen Drinks Company | Gloucestershire | |
| BPE Solicitors LLP | Gloucestershire | |
| Bridges Electrical Engineers Limited | Somerset | |
| Brightside Group plc (formerly Group Direct) | Bristol | |
| Bristol International Airport | Bristol | |
| British Falcon Plastics | Devon | |
| Budock Vean Hotel | Cornwall | |
| Burts Potato Chips Limited | Devon | |
| BV Dairy | Dorset | |
